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KMID : 0364820120480020066
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Korean Journal of Microbiology
2012 Volume.48 No. 2 p.66 ~ p.72
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Analysis of a Region Required for the Functions of Fission Yeast Nucleoporin Nup184 and Its SUMO Modification
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Chai Ai-ree
Jang Soo-Yeon
Yoon Jin-Ho
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Abstract
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The Nup188 protein is one of the largest evolutionally conserved nucleoprins (Nups) that compose the inner ring of
nuclear pore complex (NPC). The Nup184 protein, fission yeast Schizosaccharomyces pombe ortholog of Nup188p,
is required for normal growth and mRNA export in nutrient-rich medium (YES). Here, we identified a carboxyl
region (482 to 1628) of Nup184 protein that was enough to complement the defects of both growth and mRNA
export when the Δnup184 knock-out mutant was grown in YES medium. This region is also required for localization
of GFP-Nup184 fusion to the nuclear periphery. In addition, we found that ORF of Nup184 (predicted 1564
amino-acid protein) registered in S. pombe GeneDB (hosted by Sanger Institute, UK) is 64 amino-acid residues
shorter than that predicted by our sequence data. This carboxy-terminal region is necessary for the functions of
Nup184p. We further demonstrated that Nup184 protein was conjugated with SUMO in vivo.
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KEYWORD
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S. pombe, mRNA export, nucleoporin, Nup184, SUMO modification
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